VS-5584 was separated via an Acquity UPLC BEH C18 column, with a mobile phase composed of acetonitrile and 0.2% formic acid in water (40 : 60). 1.7m Fortis C18 vs 1.7m Acquity BEH C18 1.7m Fortis C18 vs 2.6m Kinetex C18 For same retention, use shorter column length = Less backpressure For longer retention, use same column length = High peak capacity / resolution 50x3.0mm .8ml/min 50x3.0mm .8ml/min 30x3.0mm .8ml/min 175bar N=169,000 1.145mins 315bar 1.95mins 230bar 1 . Green Coffee Extract (Chlorogenic Acid and Antioxidants) Conditions for all columns unless otherwise noted: Column: Kinetex 2.6m EVO C18 ACQUITY BEH 1.7m C18 . Using the ACQUITY UPLC columns calculator, methods can be fully . SiliaChrom HPLC Column: Set the flow rate (20 to 50% of the usual one) Rinse with 2-3 column volumes of each of the following solvents; Backflush the column; Set the flow rate (20 to 50% of the usual one) Rinse with 10 column volumes of each of the following solvents; Reversed-Phase Columns (C18, C18-300, C8, C8-300, C4, C4-300, Phenyl, PFP . The extracted pigments were separated and analyzed on a Waters UPLC H-Class system (Waters, Milford, USA) equipped with an Acquity UPLC BEH C18 column (1.7 m, 2.1 150 mm) (Waters, Milford, USA). Fast Analgesics Separations Over-the-counter analgesics are designed purely for pain relief. Column: 2.1 by 30mm 1.7 mm ACQUITY UPLC BEH C18 @ 358C. The active pharmaceutical ingredient was extracted from . Method transfer kits are designed to preserve the integrity of a separation as it is transferred between UPLC and HPLC platforms. A large F s factor indicates that two columns are very different. The 1.7 m ACQUITY UPLC BEH C18 Columns are a universal C18 column choice, suitable for a diverse range of analytes. In some cases the recommended comparable columns may give slightly different selectivity, and may lead to improved and more reliable separation.
Shim-pack Scepter columns consist of an organic silica hybrid particle for excellent stability and performance under a wide range of LC conditions. Equivalent Columns Our Separation Methods Technologies, Inc. recommended comparable columns will most likely give a similar selectivity. Acquity BEH C8 or C18 or equivalent) which possesses extremely low silanol activity. Sub-2 m columns are implemented for high ef-ficiency but even so, it was not enough to separate the compounds analyzed in this study.
Resolutions between bambuterol hydrochloride and montelukast sodium were found to be more than 31. Below you will find a list of columns based on manufacturer and the Waters Column recommendation.
Are you referring to a Waters Symmetry column? The AQUASIL C18 column can be run in 100% aqueous mobile phase conditions (Figures 3 and 4) and shows no tendency towards phase collapse.
If you have a "C18" column, you can be pretty sure that it actually is a plain vanilla C18 column. eluted on an Acquity BEH C18 column.
Experimental conditions: Waters Acquity UPLC BEH C18 column (50 mm2.1 mm, with 1.7 m hybrid silica particles), using a mobile phase gradient with 0.1% aqueous phosphoric acid and acetonitrile in 4 min, at a flow rate of 0.6 mL/min, with an injection volume of 1 L and detection wavelength at 220 nm. The flow price was 0.3 mL/min as well as the temperature on the column was set at 55 C. By using the volatile buffers with an equivalent ammonium level as mobile phases, we comprehensively demonstrated the effects of the elution pH value on the retention behaviors of BAs on both the BEH C18 column and HSS T3 column. Weight equivalent to 1 tablet was transferred into a 100 mL volumetric flask, 50 mL of diluent was added and sonicated for 25 min, further, the volume . This ligand, combined with the same proprietary endcapping processes as the ACQUITY UPLC BEH C18 and C8 columns, provides ultra-low column bleed, long column lifetimes and excellent peak shape. "Getting Started with ACQUITY UPLC BEH HILIC Columns" or "Getting Started with ACQUITY UPLC Amide Columns" for additional information.
However, they often show tailing or poor peak shapes due to the secondary interaction from their embedded functional groups. The mobile phase was a .
Waters ACQUITY BEH 1.7 m C18 Kinetex 2.6 m EVO C18 50 x 2.1 mm 401 Bar 50 x 2.1 mm 192 Bar Flow Rate: 0.4 mL/min Temperature: 22 C Detection: UV @ 210 nm Sample: 1. It was also seen that the larger particle size Chromatographic separation was achieved on a Waters Acquity BEH C18 (100 mm 2.1 mm, 1.7 m) column. ACQUITY UPLC BEH C 18 18 18, 130 , OBD Prep, [5 and 10 m] ACQUITY UPLC BEH C 8 8 8, 130 . Recommended GL Sciences equivalent for Waters HPLC C18 Columns: Not able to find a solution? Green Coffee Extract (Chlorogenic Acid and Antioxidants) Conditions for all columns unless otherwise noted: Column: Kinetex 2.6m EVO C18 ACQUITY BEH 1.7m C18 . However, when priming seeds with SBE, a small but significant . Can help you transition from HPLC temperature-controlled HPLC methods to UPLC methods 1.7m ACQUITY BEH C18 30! Click here to request help.
The C8 and CN columns showed a bad resolution for PAR, tailing between ACF and TIZ, and long retention time for NIM as well. Article number: 47159. Based on the concept of maintaining column length [L] to particle size [dp] ratio [L/dp], these kits provide an ACQUITY UPLC column with an HPLC column of equivalent selectivity and resolving power. seamusmoroney. Jun 30, 2022 Save as PDF Share Article number: 235716 ENVIRONMENT Column care and use Column specifications ACQUITY BEH300 C18 ANSWER The maximum back pressure is 18,000 psi. ! Using the ACQUITY UPLC columns calculator, methods can be fully . ACQUITY UPLC BEH C18 Waters 130 185 18 Hypersil GOLD (1.9m) 4-026 ACQUITY UPLC BEH C8 Waters 130 185 13 Hypersil GOLD C8 (1.9m) 4-027 ACQUITY UPLC BEH Phenyl Waters 130 185 15 Hypersil GOLD Phenyl (1.9m) 4-031 ACQUITY UPLC HSS T3 Waters 100 230 11 Hypersil GOLD aQ (1.9m) 4-029 Aeris PEPTIDE XB-C18 Phenomenex 100 200 - Accucore 150-C18 . The two fully porous sub-2 m columns evaluated, ACQUITY BEH C18 and ACQUITY CSH C18, were each only able to resolve one of the critical pairs. This application brief provides insights on how enhancements made to instrument design can improve performance, robustness, ease-of-use, or a combination of these attributes. Phases, the BEH particle & # x27 ; Rotary Tray not &. Waters ACQUITY BEH 1.7 m C18 Kinetex 2.6 m EVO C18 50 x 2.1 mm 401 Bar 50 x 2.1 mm 192 Bar Flow Rate: 0.4 mL/min Temperature: 22 C Detection: UV @ 210 nm Sample: 1. Because of the extra carbons, C18 has a larger surface area that the mobile phase has to travel across. That's a good example . Component resolution is equivalent to separations obtained using traditional capillary gel electrophoresis (CGE) methods.
Techniques Used: High Performance Liquid Chromatography. The wide-pore (300) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. Save as PDF.
The LC program was equipped with an Acquity UPLC(BEH C18, 1.7 , two.1 one hundred mm) column (Waters Associates). Column: Waters Acquity BEH C18 1.7m, 2.1 x 100 mm Column Temperature: 60 C Mobile Phase: Gradient Solvent 1: Aqueous Solution Solvent 2: Organic Solution Gradient: Time(min) The UPLC was performed on Waters ACQUITY UPLC H-Class system equipped with a Waters ACQUITY UPLC BEH C18 column (2.1 mm 50 mm, 1.7 m) with UV detection at 220 nm.
ACQUITY UPLC BEH C18 ACQUITY UPLC BEH Shield RP18 ACQUITY UPLC HSS C18 ACQUITY UPLC HSS C18 SB ACQUITY UPLC HSS T3 ACQUITY UPLC OST C18 Accucore C18 Accucore aQ Accucore 150-C18 .
<p> Ive had a high pressure issue for over 2 months now with this method im using (waters explosive method). Method transfer kits are designed to preserve the integrity of a separation as it is transferred between UPLC and HPLC platforms. Signicant efciency loss was observed on 2.1-mm id columns even with a low dispersion ultra-high pressure liquid chromatography system. C18, C8, and C4 are all linear alkylsilane phases. They used Acquity BEH C18 and SCH 18 (100 2.1 mm, dp 1.7 m) stationary phases in the RPLC mode and Acquity BEH silica and BEH amide (100 2.1 mm, dp 1.7 m) stationary phases in the HILIC mode. ACQUITY UPLC BEH Shield RP18 columns incorporate a hydrophilic carbamate group within a C 18 chain to produce exceptional peak shape for basic compounds as well as alternate selectivity compared to straight-chain alkyl columns. The chromatographic performance of two types of coreshell particles and two fully porous particles packed in 2.1 ID 50 mm columns was investigated. What is the HPLC column chemistry equivalent to ACQUITY BEH columns? Different columns were tried including an ACQUITY UPLC BEH C18 column (100 mm 2.1 mm, 1.7 m), an ACQUITY UPLC BEH C8 column (100 mm 2.1 mm, 1.7 m), and a Zorbax SB-CN column (50 4.6 mm, 1.8 m). However, the RP18 Shield gave N=30'000. Acquity BEH C 18, 130,1.7m U-VDSpher PUR 100 C18-M-SE, 1.8m use only in range of pH = 2 to 10 higher surface area and higher carbon load longer retention expected Acquity BEH C 8, 1.7m U-VDSpher PUR 100 C8-E, 1.8m use only in range of pH = 2 to 7.5 higher surface area longer retention expected Acquity BEH Phenyl, 1.7m A small F s indicates that two columns are very similar. Provides superior resolution & peak shape with best in class end-capping technology.
Kinetex C18, 3.0x100mm, 2.6um (we adapted the injection volume and flow rate) The efficiency for BEH C18 and Kinetex was similar, about 20'000 plates. Component resolution is equivalent to separations obtained . It depends . InertSustain AQ-C18 not only provide strong retention for highly . Comparisons of the performances of the EiS-150-C18 to that of the Kinetex-1.7 m-C18, Acquity-BEH-1.7 m-C18, and Zorbax-XDB-1.8 m-C18 are made and discussed. The Past 30-plus years, There are two valves, but they are the. The particle size distribution and packing . Step #2 (optional): Select F s Weighting Factors : The F s factor describes the similarity of two columns. These trifunctionally-bonded alkyl columns provide your lab with exceptional low- and high-pH stability between pH 1-12 enabling you to impact the retention, selectivity, and sensitivity of ionizable compounds. The eluted compound was detected at 254 nm with a UV detector. ENVIRONMENT Column equivalency ANSWER If the application uses a USP L1 column, the suggestion is to try an XBridge BEH C18 or ACQUITY BEH C18 column chemistry.
Compatible with 100% aqueous conditions. The 20-min gradient I on an Acquity BEH C18 column (1.7 m, 100 mm 2.1 mm) has been used in our previous . ACQUITY UPLC BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. HPLC to UPLC: Method Transfer and DevelopmentMethod Transfer From HPLC to UPLCShula Levin, Waters Israel www.forumsc. Based on the concept of maintaining column length [L] to particle size [dp] ratio [L/dp], these kits provide an ACQUITY UPLC column with an HPLC column of equivalent selectivity and resolving power. For a "RP18" column, thinghs might be trickier. Acquity BEH C8 or C18 or equivalent) which possesses extremely low silanol activity. So they have more carbons and a longer carbon chain than C8 (8 carbons) or C4 (4 carbons). porous particles (Acquity BEH) were systematically compared using a small molecule mixture and a complex natural product mixture as text probes.
What is the HPLC column chemistry equivalent to ACQUITY BEH columns? pharmaceuticals was validated as column Acquity UPLC BEH C18 (2.1 100 mm, 1.7 m) and column BEH HILIC (2.1 100 mm, . ACQUITY UPLC BEH Phenyl columns utilize a trifunctional C6 alkyl tether between the phenyl ring and the silyl functionality. Cogent e-Column C18 Columbus C18 CORTECS C18 CORTECS C18+ Cosmocore 2.6C18 Cosmosil 3C18-EB Cosmosil C18 MS-II Cosmosil C18 AR-II Cosmosil C18 AR-300 Superficially Porous Column Technologies Efficiency 90% of sub-2 m Pressure 40-50% of sub-2 m N 2X 3.5 m (totally porous) d p = 2.7m 2 m frit to reduce clogging P limit = 600 bar for HPLC or UHPLC Particles 1.7 m solid core 0.5 m diffusion path 2.7 m total diameter Poroshell 120 columns: 1.7um 0.5um 0.5um Share. There MIGHT be differences depending on the manufacturer of the column. . It is calculated according to the following equation: where: H 1 - H 2 is the difference between the hydrophobicity parameters of columns 1 and 2 S * 1 - S * 2 is the . - WKB47159 - Waters. ACQUITY UPLC Glycan BEH Columns utilize hybrid-silica BEH Technology particles functionalized with a stable, amide-containing species. Guard column . How do I determine the best column equivalent when moving from HPLC to UPLC, keeping the efficiency the same? As the premier column for method development, the 1.7 um ACQUITY UPLC BEH C18 sorbent provides you with the most universal column choice for UPLC separations. Hypersil GOLD columns Excellent peak shape for all analyte types Hypersil GOLD C8 columns Similar selectivity to C18 columns, but with reduced retention Hypersil GOLD C4 columns Similar selectivity to C18 and C8 columns, but with reduced retention The ACQUITY BEH300 C18 Peptide Separation Technology (PST) columns have been specially optimized and QC tested for peptide separations.
Shim-pack Scepter columns consist of an organic silica hybrid particle for excellent stability and performance under a wide range of LC conditions. Equivalent Columns Our Separation Methods Technologies, Inc. recommended comparable columns will most likely give a similar selectivity. Acquity BEH C8 or C18 or equivalent) which possesses extremely low silanol activity. Sub-2 m columns are implemented for high ef-ficiency but even so, it was not enough to separate the compounds analyzed in this study.
Resolutions between bambuterol hydrochloride and montelukast sodium were found to be more than 31. Below you will find a list of columns based on manufacturer and the Waters Column recommendation.
Are you referring to a Waters Symmetry column? The AQUASIL C18 column can be run in 100% aqueous mobile phase conditions (Figures 3 and 4) and shows no tendency towards phase collapse.
If you have a "C18" column, you can be pretty sure that it actually is a plain vanilla C18 column. eluted on an Acquity BEH C18 column.
Experimental conditions: Waters Acquity UPLC BEH C18 column (50 mm2.1 mm, with 1.7 m hybrid silica particles), using a mobile phase gradient with 0.1% aqueous phosphoric acid and acetonitrile in 4 min, at a flow rate of 0.6 mL/min, with an injection volume of 1 L and detection wavelength at 220 nm. The flow price was 0.3 mL/min as well as the temperature on the column was set at 55 C. By using the volatile buffers with an equivalent ammonium level as mobile phases, we comprehensively demonstrated the effects of the elution pH value on the retention behaviors of BAs on both the BEH C18 column and HSS T3 column. Weight equivalent to 1 tablet was transferred into a 100 mL volumetric flask, 50 mL of diluent was added and sonicated for 25 min, further, the volume . This ligand, combined with the same proprietary endcapping processes as the ACQUITY UPLC BEH C18 and C8 columns, provides ultra-low column bleed, long column lifetimes and excellent peak shape. "Getting Started with ACQUITY UPLC BEH HILIC Columns" or "Getting Started with ACQUITY UPLC Amide Columns" for additional information.
However, they often show tailing or poor peak shapes due to the secondary interaction from their embedded functional groups. The mobile phase was a .
Waters ACQUITY BEH 1.7 m C18 Kinetex 2.6 m EVO C18 50 x 2.1 mm 401 Bar 50 x 2.1 mm 192 Bar Flow Rate: 0.4 mL/min Temperature: 22 C Detection: UV @ 210 nm Sample: 1. It was also seen that the larger particle size Chromatographic separation was achieved on a Waters Acquity BEH C18 (100 mm 2.1 mm, 1.7 m) column. ACQUITY UPLC BEH C 18 18 18, 130 , OBD Prep, [5 and 10 m] ACQUITY UPLC BEH C 8 8 8, 130 . Recommended GL Sciences equivalent for Waters HPLC C18 Columns: Not able to find a solution? Green Coffee Extract (Chlorogenic Acid and Antioxidants) Conditions for all columns unless otherwise noted: Column: Kinetex 2.6m EVO C18 ACQUITY BEH 1.7m C18 . However, when priming seeds with SBE, a small but significant . Can help you transition from HPLC temperature-controlled HPLC methods to UPLC methods 1.7m ACQUITY BEH C18 30! Click here to request help.
The C8 and CN columns showed a bad resolution for PAR, tailing between ACF and TIZ, and long retention time for NIM as well. Article number: 47159. Based on the concept of maintaining column length [L] to particle size [dp] ratio [L/dp], these kits provide an ACQUITY UPLC column with an HPLC column of equivalent selectivity and resolving power. seamusmoroney. Jun 30, 2022 Save as PDF Share Article number: 235716 ENVIRONMENT Column care and use Column specifications ACQUITY BEH300 C18 ANSWER The maximum back pressure is 18,000 psi. ! Using the ACQUITY UPLC columns calculator, methods can be fully . ACQUITY UPLC BEH C18 Waters 130 185 18 Hypersil GOLD (1.9m) 4-026 ACQUITY UPLC BEH C8 Waters 130 185 13 Hypersil GOLD C8 (1.9m) 4-027 ACQUITY UPLC BEH Phenyl Waters 130 185 15 Hypersil GOLD Phenyl (1.9m) 4-031 ACQUITY UPLC HSS T3 Waters 100 230 11 Hypersil GOLD aQ (1.9m) 4-029 Aeris PEPTIDE XB-C18 Phenomenex 100 200 - Accucore 150-C18 . The two fully porous sub-2 m columns evaluated, ACQUITY BEH C18 and ACQUITY CSH C18, were each only able to resolve one of the critical pairs. This application brief provides insights on how enhancements made to instrument design can improve performance, robustness, ease-of-use, or a combination of these attributes. Phases, the BEH particle & # x27 ; Rotary Tray not &. Waters ACQUITY BEH 1.7 m C18 Kinetex 2.6 m EVO C18 50 x 2.1 mm 401 Bar 50 x 2.1 mm 192 Bar Flow Rate: 0.4 mL/min Temperature: 22 C Detection: UV @ 210 nm Sample: 1. Because of the extra carbons, C18 has a larger surface area that the mobile phase has to travel across. That's a good example . Component resolution is equivalent to separations obtained using traditional capillary gel electrophoresis (CGE) methods.
Techniques Used: High Performance Liquid Chromatography. The wide-pore (300) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. Save as PDF.
The LC program was equipped with an Acquity UPLC(BEH C18, 1.7 , two.1 one hundred mm) column (Waters Associates). Column: Waters Acquity BEH C18 1.7m, 2.1 x 100 mm Column Temperature: 60 C Mobile Phase: Gradient Solvent 1: Aqueous Solution Solvent 2: Organic Solution Gradient: Time(min) The UPLC was performed on Waters ACQUITY UPLC H-Class system equipped with a Waters ACQUITY UPLC BEH C18 column (2.1 mm 50 mm, 1.7 m) with UV detection at 220 nm.
ACQUITY UPLC BEH C18 ACQUITY UPLC BEH Shield RP18 ACQUITY UPLC HSS C18 ACQUITY UPLC HSS C18 SB ACQUITY UPLC HSS T3 ACQUITY UPLC OST C18 Accucore C18 Accucore aQ Accucore 150-C18 .
<p> Ive had a high pressure issue for over 2 months now with this method im using (waters explosive method). Method transfer kits are designed to preserve the integrity of a separation as it is transferred between UPLC and HPLC platforms. Signicant efciency loss was observed on 2.1-mm id columns even with a low dispersion ultra-high pressure liquid chromatography system. C18, C8, and C4 are all linear alkylsilane phases. They used Acquity BEH C18 and SCH 18 (100 2.1 mm, dp 1.7 m) stationary phases in the RPLC mode and Acquity BEH silica and BEH amide (100 2.1 mm, dp 1.7 m) stationary phases in the HILIC mode. ACQUITY UPLC BEH Shield RP18 columns incorporate a hydrophilic carbamate group within a C 18 chain to produce exceptional peak shape for basic compounds as well as alternate selectivity compared to straight-chain alkyl columns. The chromatographic performance of two types of coreshell particles and two fully porous particles packed in 2.1 ID 50 mm columns was investigated. What is the HPLC column chemistry equivalent to ACQUITY BEH columns? Different columns were tried including an ACQUITY UPLC BEH C18 column (100 mm 2.1 mm, 1.7 m), an ACQUITY UPLC BEH C8 column (100 mm 2.1 mm, 1.7 m), and a Zorbax SB-CN column (50 4.6 mm, 1.8 m). However, the RP18 Shield gave N=30'000. Acquity BEH C 18, 130,1.7m U-VDSpher PUR 100 C18-M-SE, 1.8m use only in range of pH = 2 to 10 higher surface area and higher carbon load longer retention expected Acquity BEH C 8, 1.7m U-VDSpher PUR 100 C8-E, 1.8m use only in range of pH = 2 to 7.5 higher surface area longer retention expected Acquity BEH Phenyl, 1.7m A small F s indicates that two columns are very similar. Provides superior resolution & peak shape with best in class end-capping technology.
Kinetex C18, 3.0x100mm, 2.6um (we adapted the injection volume and flow rate) The efficiency for BEH C18 and Kinetex was similar, about 20'000 plates. Component resolution is equivalent to separations obtained . It depends . InertSustain AQ-C18 not only provide strong retention for highly . Comparisons of the performances of the EiS-150-C18 to that of the Kinetex-1.7 m-C18, Acquity-BEH-1.7 m-C18, and Zorbax-XDB-1.8 m-C18 are made and discussed. The Past 30-plus years, There are two valves, but they are the. The particle size distribution and packing . Step #2 (optional): Select F s Weighting Factors : The F s factor describes the similarity of two columns. These trifunctionally-bonded alkyl columns provide your lab with exceptional low- and high-pH stability between pH 1-12 enabling you to impact the retention, selectivity, and sensitivity of ionizable compounds. The eluted compound was detected at 254 nm with a UV detector. ENVIRONMENT Column equivalency ANSWER If the application uses a USP L1 column, the suggestion is to try an XBridge BEH C18 or ACQUITY BEH C18 column chemistry.
Compatible with 100% aqueous conditions. The 20-min gradient I on an Acquity BEH C18 column (1.7 m, 100 mm 2.1 mm) has been used in our previous . ACQUITY UPLC BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. HPLC to UPLC: Method Transfer and DevelopmentMethod Transfer From HPLC to UPLCShula Levin, Waters Israel www.forumsc. Based on the concept of maintaining column length [L] to particle size [dp] ratio [L/dp], these kits provide an ACQUITY UPLC column with an HPLC column of equivalent selectivity and resolving power. For a "RP18" column, thinghs might be trickier. Acquity BEH C8 or C18 or equivalent) which possesses extremely low silanol activity. So they have more carbons and a longer carbon chain than C8 (8 carbons) or C4 (4 carbons). porous particles (Acquity BEH) were systematically compared using a small molecule mixture and a complex natural product mixture as text probes.
What is the HPLC column chemistry equivalent to ACQUITY BEH columns? pharmaceuticals was validated as column Acquity UPLC BEH C18 (2.1 100 mm, 1.7 m) and column BEH HILIC (2.1 100 mm, . ACQUITY UPLC BEH Phenyl columns utilize a trifunctional C6 alkyl tether between the phenyl ring and the silyl functionality. Cogent e-Column C18 Columbus C18 CORTECS C18 CORTECS C18+ Cosmocore 2.6C18 Cosmosil 3C18-EB Cosmosil C18 MS-II Cosmosil C18 AR-II Cosmosil C18 AR-300 Superficially Porous Column Technologies Efficiency 90% of sub-2 m Pressure 40-50% of sub-2 m N 2X 3.5 m (totally porous) d p = 2.7m 2 m frit to reduce clogging P limit = 600 bar for HPLC or UHPLC Particles 1.7 m solid core 0.5 m diffusion path 2.7 m total diameter Poroshell 120 columns: 1.7um 0.5um 0.5um Share. There MIGHT be differences depending on the manufacturer of the column. . It is calculated according to the following equation: where: H 1 - H 2 is the difference between the hydrophobicity parameters of columns 1 and 2 S * 1 - S * 2 is the . - WKB47159 - Waters. ACQUITY UPLC Glycan BEH Columns utilize hybrid-silica BEH Technology particles functionalized with a stable, amide-containing species. Guard column . How do I determine the best column equivalent when moving from HPLC to UPLC, keeping the efficiency the same? As the premier column for method development, the 1.7 um ACQUITY UPLC BEH C18 sorbent provides you with the most universal column choice for UPLC separations. Hypersil GOLD columns Excellent peak shape for all analyte types Hypersil GOLD C8 columns Similar selectivity to C18 columns, but with reduced retention Hypersil GOLD C4 columns Similar selectivity to C18 and C8 columns, but with reduced retention The ACQUITY BEH300 C18 Peptide Separation Technology (PST) columns have been specially optimized and QC tested for peptide separations.